Participation of Sulfhydryl Groups in Fatty Acid Synthesis by Chloroplast Preparations

Participation of Sulfhydryl Groups in Fatty Acid Synthesis by Chloroplast Preparations.

Among the practical problems encountered in the study of acetate metabolism by chloroplasts (23), was the fact that chloroplast preparations rapidly lost their ability to catalyze the incorporation of acetate into fatty acids. This problem has prevented the fractionation and purification of the system. With a view to stabilizing the chloroplast system, an investigation was undertaken to determi...

Studies on the mechanism of fatty acid synthesis. XI. The product of the reaction and the role of sulfhydryl groups in the synthesis of fatty acids.

Recent communications from this laboratory (1, 2) have described the preparation and properties of a highly purified enzyme fraction from avian liver which catalyzes the synthesis of long chain fatty acids from acetyl coenzyme A (CoA) and malony-Coh in the presence of reduced triphosphopyridine nucleotide (TPNH). The major component of the synthesized fatty acids formed from acetyl-Coa and malo...

C Amino Acid Incorporation by Spinach Chloroplast Preparations.

Fatty acid synthesis by enzyme preparations of Clostridium kluyveri; a consideration of postulated 4-carbon intermediates in butyrate synthesis.

Reaction 1 was observed in cell-free enzyme preparations of C. kluyveri (21), but the participation of the remaining reactions is supported only by indirect evidence obtained from studies on other bacteria (2). Koepsell et al. (12) demonstrated the formation of butyryl phosphate from acetyl phosphate and butyrate by cell-free extracts of Clostridium butylicum; and Lipmann (13, 14) demonstrated ...

Fatty Acid Synthesis by Elongases in Trypanosomes

All eukaryotic and prokaryotic organisms are thought to synthesize fatty acids using a type I or type II synthase. In addition, eukaryotes extend pre-existing long chain fatty acids using microsomal elongases (ELOs). We have found that Trypanosoma brucei, a eukaryotic human parasite that causes sleeping sickness, uses three elongases instead of type I or type II synthases for the synthesis of n...